Practice questions Salivary gland pathology

Practice questions
Salivary gland pathology
Updated: 02/21/2019
© Jun Wang, MD, PhD

1. A 67-year-old man presents with pain and swelling of left neck for 2 days. He does not have fever. He was diagnosed with squamous cell carcinoma of lung 3 years ago. Other past medical history including diabetes and hypertension. He has a 45 pack year history of cigarette smoking, and drinks wines 2 glasses a day for 30 years. Physical examination reveals a 4 cm mobile firm lesion at his left submandibular area. Sonographic examination reveals an enlarged submandibular glands. Biopsy reveals mixed serous and mucinous glands with neutrophilic infiltrate and focal abscess formation. No cytological atypia is noted. What is the diagnosis?
A. Acute sialadenitis
B. Mucinous carcinoma
C. Mucoepidermoid carcinoma
D. Sjogren syndrome
E. Squamous cell carcinoma

2. Use this case for the next two questions. A 64-year-old man presents with a slowly growing firm mass at his right upper neck for 3 months. He has a history of oral cavity leukoplakia. He smokes one and a half pack of cigarette per day for 40 years and drinks wine occasionally. Physical examination reveals a firm mass at his right submandibular area. No lymph adenopathy is noted. His oral mucosa is unremarkable, although he has poor oral hygiene. His laboratory tests are unremarkable. Image studies reveals asymmetrically enlarged right submandibular glands. Biopsy reveals diffuse fibrosis with focal lymphoplasmacytic infiltrate. Scattered acini are seen. No atypia is noted. What is the most likely the diagnosis?
A. Acute sialadenitis
B. IgG4 related dacryoadenitis and sialadenitis
C. Mucoepidermoid carcinoma
D. Sjogren syndrome
E. Squamous cell carcinoma

3. A 64-year-old man presents with a slowly growing firm mass at his right upper neck for 3 months. He has a history of oral cavity leukoplakia. He smokes one and a half pack of cigarette per day for 40 years and drinks wine occasionally. Physical examination reveals a firm mass at his right submandibular area. No lymph adenopathy is noted. His oral mucosa is unremarkable, although he has poor oral hygiene. His laboratory tests are unremarkable. Image studies reveals asymmetrically enlarged right submandibular glands. Biopsy reveals diffuse fibrosis with focal lymphoplasmacytic infiltrate. Scattered acini are seen. No atypia is noted. What serum marker is likely to be elevated?
A. Anti-nuclear antibodies
B. Anti-SSA
C. IgG4
D. Monoclonal IgG
E. Rheumatoid factor


4. Use this case for the next two questions. A 34-year-old woman presents with bilateral face swelling for 6 months. She has a history of dental caries, oral candidiasis and dry eyes. She denies history of diabetes. She does not drink alcohol nor smoke cigarette. Physical examination reveals mild nontender swelling of both parotid. No lymphadenopathy is noted. Her oral mucosa is unremarkable. Her conjunctiva has dilated vessels with reduced tear production by Schirmer test. Laboratory tests, including glucose and IgG4, are within normal range. Biopsy of the parotid reveals diffuse fibrosis, focal lymphocytic infiltrate and reduced number of acini. What is most likely the diagnosis?
A. Acute sialadenitis
B. IgG4 related dacryoadenitis and sialadenitis
C. Pleomorphic adenoma
D. Sjogren syndrome
E. Squamous cell carcinoma

5. A 34-year-old woman presents with bilateral face swelling for 6 months. She has a history of dental caries, oral candidiasis and dry eyes. She denies history of diabetes. She does not drink alcohol nor smoke cigarette. Physical examination reveals mild nontender swolling of both parotid. No lymphadenopathy is noted. Her oral mucosa is unremarkable. Her conjunctiva has dilated vessels with reduced tear production by Schirmer test. Laboratory tests, including glucose and IgG4, are within normal range. Biopsy of the parotid reveals diffuse fibrosis, focal lymphocytic infiltrate and reduced number of acini. What serum test is likely to be abnormal?
A. Anti-SSA
B. IgG4
C. IgE
D. Immunofixation
E. Rapid plasma reagin


6. A 5-year-old boy presents with a vesicle at his lower lip. His past medical history is unremarkable. Physical examination reveals a 0.5 cm vesicle with clear fluid contents. Biopsy reveals an intrastromal collection of mucin like material with neutrophilic infiltrations. No cytological atypia is seen. What is the diagnosis?
A. Bullous pemphigoid
B. Dermatitis herpetiformis
C. Herpes
D. Lichen planus
E. Mucocele

7. Use this case for the next two questions. A 31-year-old woman presents with left face mass for 1 year. The mass has been slowly growing. Her past medical history is unremarkable. Physical examination reveals a lobulated firm mass at the left face at the preauricular area. Neurological examination is unremarkable. Image studies reveal a 3.5 cm well defined parotid mass. The mass is resected. Microscopically, it has glandular components lined by short cuboid cells. The stroma is predominantly fibrotic, with foci of cartilage and bone formation. No cytological atypia nor necrosis is seen. What is the diagnosis?
A. IgG4 related dacryoadenitis and sialadenitis
B. Mucoepidermoid carcinoma
C. Pleomorphic adenoma
D. Sjogren syndrome
E. Warthin’s tumor

8. A 31-year-old woman presents with left face mass for 1 year. The mass has been slowly growing. Her past medical history is unremarkable. Physical examination reveals a lobulated firm mass at the left face at the preauricular area. Neurological examination is unremarkable. Image studies reveal a 3.5 cm well defined parotid mass. The mass is resected. Microscopically, it has glandular components lined by short cuboid cells. The stroma is predominantly fibrotic, with foci of cartilage and bone formation. No cytological atypia nor necrosis is seen. Mutation of what gene is likely associated with these findings?
A. APC
B. CDH-1
C. P53
D. PLAG1
E. RET


9. A 65-year-old woman presents with left face mass for a month. She has difficulty closing her left eye since a week ago. She has a history of recurrent pleomorphic adenoma that was treated with surgery when she was 31, 39 and 45. Image studies reveal a 2 cm mass with irregular border. Biopsy reveals predominantly fibrous tissue with foci of lymphocytic infiltrate. A few cords of markedly atypical cells are seen. Per immunohistochemistry, these cells are positive for cytokeratin. What is the most likely diagnosis?
A. Adenoid cystic carcinoma
B. Carcinoma ex pleomorphic adenoma
C. IgG4 related dacryoadenitis and sialadenitis
D. Recurrent pleomorphic adenoma
E. Warthin tumor

10. A 56-year-old man presents with slowly growing painless left face mass for 6 months. He does not have fever or abnormal facial sense or movement. He has a history of Hodgkin lymphoma 20 years ago, that was treated with radiation therapy and chemotherapy. He has a 40 pack-year history of smoking cigarette, and has been a social drinker for 30 years. Physical examination reveals a vaguely defined mass of his left preauricular area. His oral cavity is unremarkable. No lymphadenopathy is noted. Image studies reveal a 2.5 cm parotid mass. The mass is removed. Grossly, it is a cystic lesion containing brown fluid. Microscopically, it has finger-like projects convered by double layered epithelial cells with eosinophilic granular cytoplasm. The stroma has dense lymphoplasmacytic infiltrate with vague follicular architecture. No atypical cells are seen. What is the diagnosis?
A. Adenoid cystic carcinoma
B. IgG4 related dacryoadenitis and sialadenitis
C. Follicular lymphoma
D. Recurrent Hodgkin lymphoma
E. Warthin tumor

11. Use this case for the next two questions. A 71-year-old man presents with painless swelling of left preauricular area for a year. He has a history of oral erythroplakia that was monitored and stage 1 colon adenocarcinoma, that was treated with surgery. He smokes cigarette 1 pack a day for 50 years, but not drink alcohol. Physical examination reveals a firm left face mass with vague lobulated surface. No ulcer is noted. His oral mucosa has an approximately 1.5 cm erythematous area at the left posterior hard palate. Biopsy of the mass reveals irregular glands, some are covered by cells with abundant pale cytoplasm and basally located small round nuclei. Focally there are nests of slightly atypical cells with eosinophilic cytoplasm and intercellular bridges. What is the diagnosis?
A. Adenoid cystic carcinoma
B. Metastatic adenocarcinoma of colon
C. Mucoepidermoid carcinoma
D. Squamous cell carcinoma
E. Warthin’s tumor

12. A 71-year-old man presents with painless swelling of left preauricular area for a year. He has a history of oral erythroplakia that was monitored and stage 1 colon adenocarcinoma, that was treated with surgery. He smokes cigarette 1 pack a day for 50 years, but not drink alcohol. Physical examination reveals a firm left face mass with vague lobulated surface. No ulcer is noted. His oral mucosa has an approximately 1.5 cm erythematous area at the left posterior hard palate. Biopsy of the mass reveals irregular glands, some are covered by cells with abundant pale cytoplasm and basally located small round nuclei. Focally there are nests of slightly atypical cells with eosinophilic cytoplasm and intercellular bridges. Abnormality of what gene is likely associated with these findings?
A. APC
B. MECT1
C. PLAG1
D. RB1
E. RET


13. Use this case for the next three questions. A 52-year-old woman presents with painless swelling of left posterior hard palate for a year. She has a history of invasive lobular carcinoma of right breast with left axillary lymph node metastasis 5 years ago, that was treated with surgery and chemotherapy. She does not smoke cigarette, and is a social drinker. Physical examination reveals a 2 cm reddish flat hard palate mass without ulceration. Biopsy of the lesion reveals cords and nests of cells with eosinophilic cytoplasm and moderately irregular nuclei. Focally there are scattered cells with pale cytoplasm and basally located small nuclei. What is most likely the diagnosis?
A. Adenoid cystic carcinoma
B. Metastatic lobular carcinoma
C. Mucoepidermoid carcinoma
D. Squamous cell carcinoma
E. Warthin tumor

14. A 52-year-old woman presents with painless swelling of left posterior hard palate for a year. She has a history of invasive lobular carcinoma of right breast with left axillary lymph node metastasis 5 years ago, that was treated with surgery and chemotherapy. She does not smoke cigarette, and is a social drinker. Physical examination reveals a 2 cm reddish flat hard palate mass without ulceration. Biopsy of the lesion reveals cords and nests of cells with eosinophilic cytoplasm and moderately irregular nuclei. Focally there are scattered cells with pale cytoplasm and basally located small nuclei. What additional stain is likely to be confirmative for the diagnosis?
A. E-cadherin
B. Estrogen receptor
C. Mucin
D. p16
E. Silver

15. A 52-year-old woman presents with painless swelling of left posterior hard palate for a year. She has a history of invasive lobular carcinoma of right breast with left axillary lymph node metastasis 5 years ago, that was treated with surgery and chemotherapy. She does not smoke cigarette, and is a social drinker. Physical examination reveals a 2 cm reddish flat hard palate mass without ulceration. Biopsy of the lesion reveals cords and nests of cells with eosinophilic cytoplasm and moderately irregular nuclei. Focally there are scattered cells with pale cytoplasm and basally located small nuclei. Abnormality of what gene is likely associated with these findings?
A. APC
B. CDH-1
C. MECT1
D. PLAG1
E. RET


16. Use this case for the next three questions. A 42-year-old woman presents with a slowly growing swelling of her left hard palate for 6 months. She developed difficulty in mastication 2 weeks ago, but denies other symptoms. Her past medical history is unremarkable. She has a 15 pack-year history of cigarette smoking, and has been chewing betel nuts since 18. Physical examination reveals a 1.5 flat firm raised area at the left palate. This lesion has a reddish surface without ulceration. Biopsy of the lesion reveals cords and nests of epithelioid cells with round nuclei and scant cytoplasm. Focally there are round spaces between these cells. Per immunohistochemistry studies, these cells are positive for cytokeratin and negative for CD45 and CD56. Mucin stain is negative. What is the diagnosis?
A. Adenoid cystic carcinoma
B. Mucoepidermoid carcinoma
C. Small cell carcinoma
D. Small lymphocytic lymphoma
E. Squamous cell carcinoma

17. A 42-year-old woman presents with a slowly growing swelling of her left hard palate for 6 months. She developed difficulty in mastication 2 weeks ago, but denies other symptoms. Her past medical history is unremarkable. She has a 15 pack-year history of cigarette smoking, and has been chewing betel nuts since 18. Physical examination reveals a 1.5 flat firm raised area at the left palate. This lesion has a reddish surface without ulceration. No lymphadenopathy is noted. Biopsy of the lesion reveals cords and nests of epithelioid cells with round nuclei and scant cytoplasm. Focally there are round spaces between these cells. Per immunohistochemistry studies, these cells are positive for cytokeratin and negative for CD45 and CD56. Mucin stain is negative. What is most likely causing her difficulty in mastication?
A. Mandibular damage
B. Masseter inflammation
C. Paraneoplastic syndrome
D. Perineural invasion
E. Temporomandibular joint damage


18. Use this case for the next three questions. A 56-year-old man presents with a painless right preauricular mass for 3 months. His past medical history is unremarkable. He has a 30 pack-year history of cigarette smoking and alcohol abuse. He is currently seeing counseling for his alcohol addiction. Physical examination reveals a vague firm mass in his right face. Image studies reveals a 2.1 cm well defined parotid mass. A 0.5 cm lymph node is noted at the right inferior parotid edge. Biopsy of the parotid mass reveals sheets of cells with basophilic granular cytoplasm and small round nuclei. Per immunohistochemistry studies, these cells are positive for cytokeratin and alpha amylase, but negative for CD56. Mucin stain is negative. What is most likely the diagnosis?
A. Acinic cell carcinoma
B. Adenoid cystic carcinoma
C. Carcinoid
D. Mucoepidermoidl carcinoma
E. Small cell carcinoma

19. A 56-year-old man presents with a painless right preauricular mass for 3 months. His past medical history is unremarkable. He has a 30 pack-year history of cigarette smoking and alcohol abuse. He is currently seeing counseling for his alcohol addiction. Physical examination reveals a vague firm mass in his right face. Image studies reveals a 2.1 cm well defined parotid mass. A 0.5 cm lymph node is noted at the right inferior parotid edge. Biopsy of the parotid mass reveals sheets of cells with basophilic granular cytoplasm and small round nuclei. Per immunohistochemistry studies, these cells are positive for cytokeratin and alpha amylase, but negative for CD56. Mucin stain is negative. What additional marker is likely to be positive for these cells?
A. Alpha-1-antichymotrypsin
B. Chromogranin
C. CD20
D. IgG4
E. P53

20. A 13-year-old boy presents with a nodular mass on his lower lip for 6 months. He denies other symptoms. His past medical history is unremarkable. Physical examination reveals a dark red firm mass at the left lower lip. The surface mucosa appear to be normal. No lymphadenopathy is noted. Image studies reveals a 0.9 cm well defined mass. The mass is removed. Microscopically it has sheets of cells with abundant basophilic granular cytoplasm. Focally there small round spaces between these cells. Per immunohistochemistry, these cells are positive for alpha-1-antichymotrypsin and cytokeratin. What is most likely the diagnosis?
A. Acinic cell carcinoma
B. Adenoid cystic carcinoma
C. Mucoepidermoid carcinoma
D. Ranula
E. Small cell carcinoma



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